Motif confirmation by HMMer
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
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HMM file: /home/niguts/usr02/tshini/ykclst/db/Pfam_fs
Sequence file: hmm1/seq/B0218.1
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Query: B0218.1 CE06684 amidase status:Partially_confirmed TR:Q17449 protein_id:AAB00667.1
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
Amidase Amidase 595.6 3e-175 1
HAMP HAMP domain 5.2 7.4 1
Biotin_carb_C Biotin carboxylase C-terminal domain 4.9 5.1 1
Polysacc_synt Polysaccharide biosynthesis protein 4.0 6.4 1
EMP24_GP25L emp24/gp25L/p24 family 3.8 6.2 1
PS_pyruv_trans Polysaccharide pyruvyl transferase 3.3 4.8 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
EMP24_GP25L 1/1 1 30 [. 1 37 [. 3.8 6.2
HAMP 1/1 1 50 [. 1 54 [. 5.2 7.4
PS_pyruv_trans 1/1 36 56 .. 392 412 .] 3.3 4.8
Biotin_carb_C 1/1 283 302 .. 86 105 .. 4.9 5.1
Polysacc_synt 1/1 407 431 .. 271 296 .] 4.0 6.4
Amidase 1/1 84 559 .. 1 521 [] 595.6 3e-175
Alignments of top-scoring domains:
EMP24_GP25L: domain 1 of 1, from 1 to 30: score 3.8, E = 6.2
*->mlslllllalvtsvdsaltFylpagekkEqvCfyedl<-*
++ l++lv+ + +a+++++ +++kk ++e+l
B0218.1 1 --MIFYLVLLVLGA-IAFYVHFSNNRKK----LIERL 30
HAMP: domain 1 of 1, from 1 to 50: score 5.2, E = 7.4
*->lllvlllalllalllalllarrltrPlrrLaeaaqriasGrgGdltv
+++ l+l++l+a+++++ ++++ + ++rL+ +aqr + +dl++
B0218.1 1 MIFYLVLLVLGAIAFYVHFSNNRKKLIERLEIVAQRRR----DDLSK 43
rlpvsgr<-*
+++ ++
B0218.1 44 NVEQARK 50
PS_pyruv_trans: domain 1 of 1, from 36 to 56: score 3.3, E = 4.8
*->ekettlskrveevrksalknf<-*
+++++lsk ve++rk+a+k +
B0218.1 36 RRRDDLSKNVEQARKAADKLD 56
Biotin_carb_C: domain 1 of 1, from 283 to 302: score 4.9, E = 5.1
*->evkTNIpFLialLedpdFls<-*
+vkTN++FL+++ d dF+s
B0218.1 283 DVKTNVEFLRNVWGDIDFQS 302
Polysacc_synt: domain 1 of 1, from 407 to 431: score 4.0, E = 6.4
*->kllvillsliasivtavlfPrfsklq<-*
++ v ++++++++v+ +fPr+ +l+
B0218.1 407 MVPVWIQRILSYPVS-LVFPRMGMLM 431
Amidase: domain 1 of 1, from 84 to 559: score 595.6, E = 3e-175
*->ElveaflarieaanpklNvtafvtvffeeAlaaAkaadkrrarkrgg
E+++a+ +++ a++k+N +vt+f+ +A+++A+++d++++ + +
B0218.1 84 EAIRAYFHKAILAHEKTN---AVTCFILDAERQAEELDEQAK-LPYY 126
ekgPLhGvPialKDnidvkGvpTTaGskalegypppyDAtvVerLraaGA
k+PL+GvP++lK+++ vkG++TT+G+ + +++p+++D+ Ve ++++G
B0218.1 127 VKPPLFGVPLSLKECLKVKGYDTTRGFVQDAYHPATEDSIQVEHYKKLGL 176
vilGKTNmdEfamgsttensafGpTrNPwdlsrtpGGSSGGSAAaVAagl
+++++TN++++++++ ++n+ fG+T NP+d +rt+GGSSGG++A+++ag+
B0218.1 177 IPFCQTNVPQSLLSYNCSNPLFGTTTNPYDSTRTCGGSSGGEGALIGAGG 226
vplAiGtDTGGSIRiPAafCGlvGlKPTyGRvSryGvvgSveplssslDq
++++iGtD+GGS+RiP++f+G++G+KP+ R+ ++G +Sv++ + + +
B0218.1 227 SLIGIGTDVGGSVRIPCHFTGTAGIKPSKMRFAHRGGGASVPGKPLIDAN 276
vGPlARsVeDaAllLdviaGydpgDptsadsppPtvpwdpvpdyaeglkk
GP+A++V+ ++ +L+ ++G+ + ++ ++++pP v+w+++ +++
B0218.1 277 DGPMAKDVKTNVEFLRNVWGDIDFQSDRDPYCPP-VHWNES------VYS 319
dlkgLRiGvpredfgddLdhVaActIRLdgldfvEaDipialnyasldpe
++k+LR+G++ +d+ +++++p+
B0218.1 320 SEKKLRVGYYIDDG-----------------------------WFTPTPA 340
vdeavekaaaaLeelGhhevvevslpslkealplyyliapaeassnlary
+++av++ ++ Le++G h+v+++ +p+l++ ++ly+ ++ +++++++++
B0218.1 341 LQRAVLESKKHLEAAG-HTVIPFYPPRLPSVMQLYFRAVCLDGGQYVLNK 389
dGvryGergleddeasdldelyprtraeglgdevkrrielgayalsagys
+ +++e+ ++++ + ++ p ++++ l+ v + +++++ +++ +
B0218.1 390 LLKDIIEPTIRFQ---VTLWMVPVWIQRILSYPVSLVFPRMGMLMQSLTR 436
dayeylkaqkvrrllrrefdglFeehgvDvLisPTtptpAprigepdkli
d++e+++a+++++ +r+ef+gl+ ++++Dv+++P+ +pAp+++ p+k++
B0218.1 437 DTFELREAYADIEAYREEFVGLMMKDNLDVILCPASIMPAPQHDIPSKVV 486
seaddytvlyllddftantvPaNlaGlPaiSvPvgfspeds.wdalvk.e
++ +yt+ly+l+df a++vP++ + + ++ ++++pe+++w++++k++
B0218.1 487 -SGVSYTCLYNLLDFGAGVVPVTAVSKSDEEKLINEYPETDkWYQITKkA 535
ylpegyvGLPvGlQiiGkpgdeetLL<-*
+l g+vG+P+G+Q+ ++p++ee +L
B0218.1 536 TL--GAVGMPIGVQVAAPPYREEAVL 559