Motif confirmation by HMMer
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
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HMM file: /home/niguts/usr02/tshini/ykclst/db/Pfam_fs
Sequence file: hmm1/seq/C47E12.5
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Query: C47E12.5 CE05449 ubiquitin-activating enzyme status:Partially_confirmed TR:Q27481 protein_id:CAA93101.1
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
ThiF ThiF family 364.2 1.4e-105 2
UBACT Repeat in ubiquitin-activating (UBA) protei 161.0 2e-44 2
SAP SAP domain 6.0 6.4 1
DUF206 S-adenosylmethionine decarboxylase 5.5 1.8 1
TrkA-N TrkA-N domain 4.9 7.1 1
pyr_redox Pyridine nucleotide-disulphide oxidoreducta 2.8 4.9 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
TrkA-N 1/1 127 145 .. 1 19 [. 4.9 7.1
ThiF 1/2 124 254 .. 1 149 [] 150.6 2.7e-41
pyr_redox 1/1 518 532 .. 172 186 .. 2.8 4.9
SAP 1/1 568 588 .. 15 35 .] 6.0 6.4
ThiF 2/2 516 659 .. 1 149 [] 217.2 2.4e-61
DUF206 1/1 821 839 .. 7 26 .. 5.5 1.8
UBACT 1/2 803 841 .. 1 41 [. 15.1 0.0079
UBACT 2/2 905 977 .. 1 75 [] 147.5 1.4e-40
Alignments of top-scoring domains:
TrkA-N: domain 1 of 1, from 127 to 145: score 4.9, E = 7.1
*->hfiviGlGrvGrsvakeLl<-*
+++ GlG+vG +ak+L+
C47E12.5 127 SVLISGLGSVGVEIAKNLI 145
ThiF: domain 1 of 2, from 124 to 254: score 150.6, E = 2.7e-41
*->knskVLvvGaGGLGsealknLalaGvGsGGkltivDgDtVelsNLnR
++++VL++G+G +G+e++knL+l Gv+ ++ti+D +s+L+
C47E12.5 124 RTASVLISGLGSVGVEIAKNLILGGVR---HVTIHDTKLAKWSDLSA 167
QflfresDvGkMpKaeaAaerLqelNPdvevsvtavnerltpenaeefie
Q+++r++DvG+ ++a++++erL+elN +v+ v++ t+e +eef++
C47E12.5 168 QYYLRDADVGH-NRATSCYERLAELNDSVN--VQVS----TDELTEEFVK 210
gfqldvVvdalDnfearllingllllldaCvklgiplieagtlGlkGqvt
f d+Vv + +a+++i +++++++++++ ++++G++++++
C47E12.5 211 TF--DLVVLTDAARTAQRQIA------AWTRAHNRRILITDARGVFSYIF 252
vf<-*
+
C47E12.5 253 ND 254
pyr_redox: domain 1 of 1, from 518 to 532: score 2.8, E = 4.9
*->krvvVvGGGyIGlEl<-*
+r VvG+G IG+El
C47E12.5 518 QRWFVVGAGAIGCEL 532
SAP: domain 1 of 1, from 568 to 588: score 6.0, E = 6.4
*->krGLstsGkKaeLieRLleal<-*
+r +++GkK+e+ +R +a+
C47E12.5 568 FRRRDVGGKKSECAARAVTAF 588
ThiF: domain 2 of 2, from 516 to 659: score 217.2, E = 2.4e-61
*->knskVLvvGaGGLGsealknLalaGvGsG..GkltivDgDtVelsNL
++++++vvGaG++G+e+lknL ++Gv +G++G + i+D+D++e+sNL
C47E12.5 516 FRQRWFVVGAGAIGCELLKNLSMMGVACGegGLIKITDMDQIEISNL 562
nRQflfresDvGkMpKaeaAaerLqelNPdvevsvtavnerltpenaeef
nRQflfr+ DvG +K+e+Aa++++++N+dv+ ++a er++ e+++ f
C47E12.5 563 NRQFLFRRRDVGG-KKSECAARAVTAFNSDVR--IEALAERVGLETEHIF 609
iegfq..ldvVvdalDnfearllingllllldaCvklgiplieagtlGlk
+++f+++l+ V++alDn++ar++++ ++Cv++++pl+e+gt G+k
C47E12.5 610 NDEFFgeLNGVANALDNVDARRYMD------RRCVYYRLPLLESGTMGTK 653
Gqvtvf<-*
G+++v+
C47E12.5 654 GNTQVV 659
DUF206: domain 1 of 1, from 821 to 839: score 5.5, E = 1.8
*->iIaELyGcDpdkiddvekIr<-*
+IaELyG+ p +d++e+Ir
C47E12.5 821 LIAELYGVQP-ILDREEVIR 839
UBACT: domain 1 of 2, from 803 to 841: score 15.1, E = 0.0079
*->eFDkDDdsneHmdFvtaaAnlRAenygIpgpvdrfkikgia<-*
+FD+ eH Fv aa++l Ae yg + + dr ++ ++a
C47E12.5 803 NFDPSK--EEHFNFVFAASILIAELYGVQPILDREEVIRVA 841
UBACT: domain 2 of 2, from 905 to 977: score 147.5, E = 1.4e-40
*->eFDkDDdsneHmdFvtaaAnlRAenygIpgpvdrfkikgiagnIkIP
F+kDDdsn+Hm+F+taa+nlRAeny+I p+dr+++k+iag+I IP
C47E12.5 905 DFEKDDDSNHHMEFITAASNLRAENYDIL-PADRMRTKQIAGKI-IP 949
AiAtTNAvvAGlcclEavKvltgdadel<-*
AiAtT+A+vAGl+c+E++Kv+++ ++
C47E12.5 950 AIATTTAAVAGLVCIELYKVVDANGIPK 977