Motif confirmation by HMMer
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
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HMM file: /home/niguts/usr02/tshini/ykclst/db/Pfam_fs
Sequence file: hmm2/seq/F12F3.2a
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Query: F12F3.2a CE30753 titin status:Partially_confirmed TN:AAM29672 protein_id:AAM29672.1
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
ig Immunoglobulin domain 423.3 2.2e-123 19
pkinase Protein kinase domain 285.8 5.3e-82 1
fn3 Fibronectin type III domain 107.9 1.1e-29 2
PKD PKD domain 8.0 0.25 4
cdc48_2 Cell division protein 48 (CDC48), doma 6.1 3.7 1
DUF594 Protein of unknown function, DUF594 5.8 3.4 1
DIX DIX domain 5.5 4.1 1
TrpBP Tryptophan RNA-binding attenuator prot 4.7 5 1
UPF0058 Uncharacterised protein family UPF0058 4.1 4.2 1
SecA_protein SecA protein, amino terminal region 3.8 0.77 1
Closter_coat Closterovirus coat protein 3.5 9 1
Umbravirus_LDM Umbravirus long distance movement (LDM 3.4 3.7 1
Ribosomal_L25p Ribosomal L25p family 2.9 9.6 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
fn3 1/2 50 134 .. 1 84 [] 62.2 1.6e-16
DUF594 1/1 251 266 .. 1 16 [. 5.8 3.4
UPF0058 1/1 307 315 .. 1 13 [. 4.1 4.2
Ribosomal_L25p 1/1 309 317 .. 83 91 .] 2.9 9.6
pkinase 1/1 195 450 .. 1 294 [] 285.8 5.3e-82
ig 1/19 532 590 .. 4 50 .] 39.4 2.9e-09
DIX 1/1 688 708 .. 67 87 .] 5.5 4.1
SecA_protein 1/1 710 718 .. 430 438 .] 3.8 0.77
ig 2/19 665 719 .. 10 50 .] 12.5 0.086
TrpBP 1/1 717 731 .. 61 75 .] 4.7 5
ig 3/19 768 819 .. 14 50 .] 28.4 3.2e-06
Umbravirus_LDM 1/1 860 879 .. 213 232 .] 3.4 3.7
ig 4/19 866 925 .. 1 50 [] 43.3 2.4e-10
ig 5/19 965 1026 .. 1 50 [] 35.3 4e-08
ig 6/19 1098 1150 .. 9 50 .] 14.8 0.019
ig 7/19 1193 1255 .. 1 50 [] 35.9 2.7e-08
ig 8/19 1298 1355 .. 1 47 [. 17.9 0.0027
PKD 1/4 1355 1373 .. 80 99 .] 2.4 15
fn3 2/2 1398 1480 .. 1 84 [] 45.7 9.7e-12
ig 9/19 1520 1568 .. 13 50 .] 5.8 5.8
PKD 2/4 1570 1583 .. 85 99 .] 0.1 79
ig 10/19 1618 1679 .. 1 50 [] 34.6 6.3e-08
ig 11/19 1717 1778 .. 1 50 [] 42.4 4.4e-10
ig 12/19 1831 1893 .. 1 50 [] 16.4 0.0067
ig 13/19 1936 2002 .. 1 50 [] 20.0 0.00071
PKD 3/4 2002 2017 .. 83 99 .] 1.2 35
ig 14/19 2053 2100 .. 14 47 .. 3.4 27
cdc48_2 1/1 2204 2220 .. 1 20 [. 6.1 3.7
ig 15/19 2279 2329 .. 13 50 .] 5.0 10
ig 16/19 2382 2443 .. 1 50 [] 28.0 4.2e-06
Closter_coat 1/1 2510 2538 .. 81 110 .. 3.5 9
ig 17/19 2487 2545 .. 1 47 [. 13.9 0.035
ig 18/19 2589 2645 .. 1 46 [. 18.9 0.0014
PKD 4/4 2647 2664 .. 81 99 .] 5.0 2.2
ig 19/19 2689 2750 .. 1 50 [] 23.1 9.5e-05
Alignments of top-scoring domains:
fn3: domain 1 of 2, from 50 to 134: score 62.2, E = 1.6e-16
*->PsaPtnltvtdvtstsltlsWsppt..gngpitgYevtyRqpkngge
P aP+ + v+d++ +s++l+W +pt++g++p+ +Y+v+ +++ g
F12F3.2a 50 PAAPGKPAVEDQNVDSVRLRWAAPTndGGSPVRNYTVEM-CTEKGKT 95
wneltvpgtttsytltgLkPgteYevrVqAvnggG.GpeS<-*
w+ v++++ tl +L+Pg Y +rV+A n+ G++++S
F12F3.2a 96 WTKAEVTKQA-FITLFNLVPGESYRFRVRADNTFGqSEPS 134
DUF594: domain 1 of 1, from 251 to 266: score 5.8, E = 3.4
*->eLleleedkndgeemW<-*
+Ll+l e +++g emW
F12F3.2a 251 KLLNLHEAFDMGNEMW 266
UPF0058: domain 1 of 1, from 307 to 315: score 4.1, E = 4.2
*->MhGqFGKEELiqL<-*
Mh K ++++L
F12F3.2a 307 MH----KNQIVHL 315
Ribosomal_L25p: domain 1 of 1, from 309 to 317: score 2.9, E = 9.6
*->kreLqHiDf<-*
k+ ++H+D+
F12F3.2a 309 KNQIVHLDL 317
pkinase: domain 1 of 1, from 195 to 450: score 285.8, E = 5.3e-82
*->yelleklGeGsfGkVykakhkdktgkiVAvKilkkekesikek.rfl
y ++e+lG+G++G+Vy+a++k tgk++A K++ ++ +k++++
F12F3.2a 195 YIIHEELGKGAYGTVYRATEK-ATGKTWAAKMVQ--VRPGVKKeNVI 238
rEiqilkrLsHpNIvrligvfedtddhlylvmEymegGdLfdylrrnggp
Ei+++++L+H+ +++l+++f +++++l+ E++ gG+Lf+ + + +
F12F3.2a 239 HEISMMNQLHHEKLLNLHEAFD-MGNEMWLIEEFVSGGELFEKILEDDSL 287
lsekeakkialQilrGleYLHsngivHRDLKpeNILlden.dgtvKiaDF
+se+e++++++Qil G++++H n ivH DLKpeNILl+ ++ + +Ki+DF
F12F3.2a 288 MSEEEVRDYMHQILLGVSHMHKNQIVHLDLKPENILLKAKnSNELKIIDF 337
GLArllesssklttfvGTpwYmmAPEvilegrgysskvDvWSlGviLyEl
GLAr+l++ + + + GTp+++ APEv +++++ + D+W +Gvi y l
F12F3.2a 338 GLARKLDPKKSVKLLFGTPEFC-APEV-VNYQPVGLSTDMWTVGVISYVL 385
ltggplfpgadlpaftggdevdqliifvlklPfsdelpktridpleelfr
l+g +Pf + + ++d+l+++ +
F12F3.2a 386 LSG--------------------------LSPFLG---DSDEDTLANVSA 406
iikrpglrlplpsncSeelkdLlkkcLnkDPskRpGsatakeilnhpwf<
+++ + p ++ +S+ +kd+++++ kD kR + +++l hpw+
F12F3.2a 407 SDWD--FDDPSWDDVSDLAKDFICRLMIKDKRKRM---SVQDALRHPWI 450
-*
F12F3.2a - -
ig: domain 1 of 19, from 532 to 590: score 39.4, E = 2.9e-09
*->vtLtCsvsgfgpp.p.vtWlrngk.le...........tsslslti.
+tL+C v g p+p+v+W++++k+l+ ++ + ++ + + + lt++
F12F3.2a 532 ATLSCDVD--GVPsPkVQWYKDDKeLTvpsmkydsfynEGLAELTVk 576
svtpeDsgGtYtCvv<-*
+ ++D+ G YtC++
F12F3.2a 577 NIVESDA-GKYTCRA 590
DIX: domain 1 of 1, from 688 to 708: score 5.5, E = 4.1
*->DsarLrPcfNgrvvswLvsve<-*
Ds Lr+ gr+++ ++sv+
F12F3.2a 688 DSNYLRKHDKGRYELHILSVD 708
SecA_protein: domain 1 of 1, from 710 to 718: score 3.8, E = 0.77
*->TeeaKwkAV<-*
T+e+KwkAV
F12F3.2a 710 TDEGKWKAV 718
ig: domain 2 of 19, from 665 to 719: score 12.5, E = 0.086
*->vsgfgpp.p.vtWlrngk.le...........tsslslti.svtpeD
v++++ p+p+v W++ng+++ ++++ ++++ + + l+i sv + D
F12F3.2a 665 VTNTTLPePtVDWYHNGEhISindsnylrkhdKGRYELHIlSVDSTD 711
sgGtYtCvv<-*
+ G v
F12F3.2a 712 E-GKWKAVG 719
TrpBP: domain 1 of 1, from 717 to 731: score 4.7, E = 5
*->AliqtayGEiksEkK<-*
A+ + a+GE +sE+K
F12F3.2a 717 AVGKNAFGECESEAK 731
[output cut off at A = 10 top alignments]