Motif confirmation by HMMer
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
HMM file: /home/niguts/usr02/tshini/ykclst/db/Pfam_fs
Sequence file: hmm2/seq/F17E5.1a
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: F17E5.1a CE27131 locus:lin-2 MAGUK protein, calcium\/calmodulin dependent protein kinase status:Confirmed SW:P54936
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
Guanylate_kin Guanylate kinase 217.4 2.1e-61 1
pkinase Protein kinase domain 175.1 1.1e-48 1
L27 L27 domain 95.2 6.1e-25 2
PDZ PDZ domain (Also known as DHR or GLGF) 84.1 1.6e-22 1
SH3 SH3 domain 28.5 3.4e-06 2
NIF NLI interacting factor 6.1 1.1 1
Calcipressin Calcipressin 3.2 7 1
DUF414 Protein of unknown function, DUF414 3.0 6.5 2
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
pkinase 1/1 64 281 .. 45 294 .] 175.1 1.1e-48
L27 1/2 371 425 .. 1 56 [] 39.4 2.4e-09
L27 2/2 428 482 .. 1 56 [] 55.7 6.7e-14
DUF414 1/2 532 550 .. 180 198 .] 3.9 3.7
PDZ 1/1 546 626 .. 1 84 [] 84.1 1.6e-22
SH3 1/2 636 650 .. 1 15 [. 7.7 1.8
SH3 2/2 659 681 .. 17 39 .. 20.7 0.00047
DUF414 2/2 759 773 .. 184 198 .] 0.9 27
NIF 1/1 766 780 .. 1 15 [. 6.1 1.1
Calcipressin 1/1 801 814 .. 262 275 .] 3.2 7
Guanylate_kin 1/1 810 914 .. 1 108 [] 217.4 2.1e-61
Alignments of top-scoring domains:
pkinase: domain 1 of 1, from 64 to 281: score 175.1, E = 1.1e-48
*->flrEiqilkrLsHpNIvrligvfedtddhlylvmEymegGdL.fdyl
+ Ei+i ++L+Hp+I+rl + + + ++++E meg d f+++
F17E5.1a 64 IDEEIRICQNLQHPYICRLEKTIN-SVHYRHIIFENMEGNDIcFEIV 109
rrng..gplsekeakkialQilrGleYLHsngivHRDLKpeNILlden..
+r +++++se + ++++Q+l +l+Y+H ++ivHRD+ p N+ l ++++
F17E5.1a 110 QRASngFVFSEYVVSHYTRQLLDALDYCHTRKIVHRDVRPHNLVLASKdt 159
dgtvKiaDFGLArlle..sssklttfvGTpwYmmAPEvilegrgysskvD
+K++ FG A++l++ +s++ ++vG p +m APE+ ++ + s +D
F17E5.1a 160 SAPLKLCGFGVAKDLSeiGGSMACGRVGVPQFM-APEI-VRKDRVSCSSD 207
vWSlGviLyElltggplfpgadlpaftggdevdqliifvlklPfsdelpk
+WS Gv+L+ ll g +lPfs+
F17E5.1a 208 IWSSGVVLFLLLAG--------------------------RLPFSG---- 227
tridpleelfriikrpglrlplp.sncSeelkdLlkkcLnkDPskRpGsa
++ + + ri ++ + + + +n+Se+++ L++++Ln DPskR+
F17E5.1a 228 ---STSDIYERIMQT-DVDVDGYmPNISESARNLVRRMLNADPSKRI--- 270
takeilnhpwf<-*
+ake+lnh w+
F17E5.1a 271 SAKEALNHEWI 281
L27: domain 1 of 2, from 371 to 425: score 39.4, E = 2.4e-09
*->avqrllelLeeLqslseaseedlaeLrklLqsphlqaLlevHDkvae
+v ++l+++++L ++++++ +++ ++k++++ ++++Ll+++Dk+++
F17E5.1a 371 KVLGSLDAINSLLDPNSYK-PGSTTFQKIHDDGSVRNLLRLYDKIKA 416
kvydpplpe<-*
++p+ e
F17E5.1a 417 LPCEPVVTE 425
L27: domain 2 of 2, from 428 to 482: score 55.7, E = 6.7e-14
*->avqrllelLeeLqslseaseedlaeLrklLqsphlqaLlevHDkvae
+ + +e+L+++++l+++s +++ eLr+lL+sphl +++++ D+v++
F17E5.1a 428 TSTLRKETLNQIDGLLGPS-PEALELRQLLNSPHLASCVQALDVVVC 473
kvydpplpe<-*
+++dp++++
F17E5.1a 474 EIRDPKNEA 482
DUF414: domain 1 of 2, from 532 to 550: score 3.9, E = 3.7
*->eeEdDllrtfEkldinqfr<-*
eeE++l+ +l++ qf+
F17E5.1a 532 EEEEELYDCMSRLRLVQFQ 550
PDZ: domain 1 of 1, from 546 to 626: score 84.1, E = 1.6e-22
*->evtlekevkrgglGfsikggsdkgivvsevlpGsgaAeagGrLkeGD
+v+++k+ +++++G+++k+++d++++v+++++G g+ +++ L++GD
F17E5.1a 546 LVQFQKD-TQEPMGITLKVNEDGRCFVARIMHG-GMIHRQATLHVGD 590
vIlsvNGqdvenmsheravlaikgsggsevtLtvlRd<-*
I ++NG +v+n s+e +++++ ++g vt ++++
F17E5.1a 591 EIREINGMSVANRSVESLQEMLRDARG-QVTFKIIPS 626
SH3: domain 1 of 2, from 636 to 650: score 7.7, E = 1.8
*->pkvvALYDYeaeesd<-*
+v+A +DYe++++d
F17E5.1a 636 IFVRAQFDYEPSQDD 650
SH3: domain 2 of 2, from 659 to 681: score 20.7, E = 0.00047
*->LsFkkGDvitvleksddgWWkgr<-*
++Fk GD+++v++k+d +WW +r
F17E5.1a 659 IPFKTGDILQVISKDDHNWWQAR 681
DUF414: domain 2 of 2, from 759 to 773: score 0.9, E = 27
*->DllrtfEkldinqfr<-*
Dl +E +++ q+r
F17E5.1a 759 DLVTYEEVMRLSQYR 773
NIF: domain 1 of 1, from 766 to 780: score 6.1, E = 1.1
*->psrlslvqkKtLvlD<-*
+rls++++KtLvl+
F17E5.1a 766 VMRLSQYRRKTLVLL 780
Calcipressin: domain 1 of 1, from 801 to 814: score 3.2, E = 7
*->rpkakivqTarPpr<-*
r+ +i++T+rPpr
F17E5.1a 801 RFAYPIPHTTRPPR 814
[output cut off at A = 10 top alignments]