Motif confirmation by HMMer
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
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HMM file: /home/niguts/usr02/tshini/ykclst/db/Pfam_fs
Sequence file: hmm2/seq/F25H5.4
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Query: F25H5.4 CE15900 locus:eft-2 Elongation factor Tu family (contains ATP\/GTP binding P-loop) status:Confirmed SW:P29691 protein_id:CAB02
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
GTP_EFTU Elongation factor Tu GTP binding domain 297.5 1.6e-85 1
EFG_IV Elongation factor G, domain IV 177.5 2.2e-49 2
EFG_C Elongation factor G C-terminus 134.7 9e-39 1
GTP_EFTU_D2 Elongation factor Tu domain 2 69.0 6.3e-18 1
HS1_rep Repeat in HS1/Cortactin 8.3 0.21 1
Dioxygenase Dioxygenase 4.2 6.1 1
serpin Serpin (serine protease inhibitor) 3.9 2.3 1
PapG_N PapG carbohydrate binding domain 3.5 6 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
HS1_rep 1/1 243 280 .. 1 37 [] 8.3 0.21
PapG_N 1/1 334 340 .. 1 7 [. 3.5 6
GTP_EFTU 1/1 17 356 .. 1 298 [] 297.5 1.6e-85
Dioxygenase 1/1 423 431 .. 1 9 [. 4.2 6.1
serpin 1/1 443 454 .. 375 388 .] 3.9 2.3
GTP_EFTU_D2 1/1 390 480 .. 1 96 [] 69.0 6.3e-18
EFG_IV 1/2 569 576 .. 1 8 [. 1.2 56
EFG_IV 2/2 614 731 .. 1 131 [] 176.2 5.5e-49
EFG_C 1/1 733 821 .. 1 93 [] 134.7 9e-39
Alignments of top-scoring domains:
HS1_rep: domain 1 of 1, from 243 to 280: score 8.3, E = 0.21
*->GfGGkfGvqkdrvDksAvGfdyqe.kvekhes.qkDysk<-*
+ GkfGvq d+ k G + + k++k +s+q+D sk
F25H5.4 243 -YAGKFGVQVDKLMKNLWGDRFFDlKTKKWSStQTDESK 280
PapG_N: domain 1 of 1, from 334 to 340: score 3.5, E = 6
*->MKKWFPA<-*
M KW+PA
F25H5.4 334 MRKWLPA 340
GTP_EFTU: domain 1 of 1, from 17 to 356: score 297.5, E = 1.6e-85
*->krhrNigiiGHVDHGKTTLtdrLlyytgaisertleklegeakegge
+++rN+++i+HVDHGK+TLtd+L+ ++g+i +a+e
F25H5.4 17 RNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAG-------SKAGET-- 54
rkgsfklarviDkmeeEreRGITIksahvsfewkdededletkake.gkk
r++D++++E+eR+ITIks+++s++++ e++dl ++ + +
F25H5.4 55 --------RFTDTRKDEQERCITIKSTAISLFFELEKKDL----EFvKGE 92
r.gn........elTekrhiniIDTPGHvDFtkevirGlaqaDGAiLVVd
+ ++ + ++++e+ ++++in+ID+PGHvDF++ev+++l+++DGA++VVd
F25H5.4 93 NqFEtvevdgkkEKYNGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVD 142
AveGvmpGfkGQTrehlrlArqlgvppiilvVaiNKiDrvgaelklppvp
+v Gv+ QT+++lr+A ++++ +++l+ +NK+Dr+ el+l +
F25H5.4 143 CVSGVCV----QTETVLRQAIAERI-KPVLF--MNKMDRALLELQL--GA 183
eelyeelekevrellkpIg.e.df.gvidli...................
eel++ ++++v++++++I++++d++g i +++ ++ + +++ ++
F25H5.4 184 EELFQTFQRIVENINVIIAtYgDDdGPMGPImvdpsignvgfgsglhgwa 233
...........................................lae.vae
+ ++ + ++ + + ++ ++ +++ + ++++ +++++++ ++ +
F25H5.4 234 ftlkqfaemyagkfgvqvdklmknlwgdrffdlktkkwsstqtDESkRGF 283
.dd.lmeyyfegeeipiipiSalggdnidelsiknkLk.kd.dlslvPwy
++ l +++++++++++i+ +++ +++l+ik L+++++dl+ +P+
F25H5.4 284 cQFvLDPIFMVFDAVMNIKK-DKTAALVEKLGIK--LAnDEkDLEGKPLM 330
kgvSalknkgrkrgaiptLLealddylPsPe<-*
k + ++++ + +t+L+++ +lPsP+
F25H5.4 331 KVFMRKWLP----A-GDTMLQMIAFHLPSPV 356
Dioxygenase: domain 1 of 1, from 423 to 431: score 4.2, E = 6.1
*->ieGPfYvag<-*
i+GP+Yv+g
F25H5.4 423 IQGPNYVPG 431
serpin: domain 1 of 1, from 443 to 454: score 3.9, E = 2.3
*->gsiLFmGkHVtvnP<-*
++iL+mG+ + ++P
F25H5.4 443 RTILMMGR-F-IEP 454
GTP_EFTU_D2: domain 1 of 1, from 390 to 480: score 69.0, E = 6.3e-18
*->adVfkikytmDpyrrTSdGrGtVatgRvesGtlkkGdeveigg.ngt
+++ k+ +t+D++r + a+gRv+sG++ G++ +i g+n++
F25H5.4 390 MYISKMVPTSDKGR-------FYAFGRVFSGKVATGMKARIQGpNYV 429
.kk.kdvpevtrVtslemfhg.ldeavaGdnaGlivagiglkdaaikrGd
++k++d +e+t ++++m+ g+ e+++ + G+i++++g++++ + +G
F25H5.4 430 pGKkEDLYEKTIQRTILMM-GrFIEPIEDIPSGNIAGLVGVDQY-LVKGG 477
vla<-*
+++
F25H5.4 478 TIT 480
EFG_IV: domain 1 of 2, from 569 to 576: score 1.2, E = 56
*->pqVayrET<-*
p V+yrET
F25H5.4 569 PVVSYRET 576
EFG_IV: domain 2 of 2, from 614 to 731: score 176.2, E = 5.5e-49
*->pqVayrETIrkdvkereykhkkQsGgdgqyakviiriePlpsarGse
++V+ r + k+r++++++++ d+ +a++i++++P++ +G+
F25H5.4 614 GTVNAR----DEFKARAKILAEKYEYDVTEARKIWCFGPDG--TGP- 653
gGsGneFvdetkGvGGqiPkEyipavekGfreamkeGpLaGePvvdvkvt
n d tk G+q+++E++++v++Gf++a++eG+L +e++++v+++
F25H5.4 654 ----NLLMDVTK--GVQYLNEIKDSVVAGFQWATREGVLSDENMRGVRFN 697
LvDGsyHeDAVdSseaaFkpAarrAfkeAllkAk<-*
++D+++H+DA+++++++++p+arr+f++++l+A+
F25H5.4 698 VHDVTLHADAIHRGGGQIIPTARRVFYASVLTAE 731
EFG_C: domain 1 of 1, from 733 to 821: score 134.7, E = 9e-39
*->vlLEPimkveIivPeeylGaViglLnkRRGqildmeprgggaPlrvr
+lLEP++ veI++Pe +G+++g+Ln+RRG+++++ + g+ P++v
F25H5.4 733 RLLEPVYLVEIQCPEAAVGGIYGVLNRRRGHVFEESQVTGT-PMFV- 777
ikaevPlaEmfGDFatdLrSlTqGrAsfsmeFdhYepvpgviadel<-*
+ka++P++E+fG F+ dLrS T G+A+++++Fdh++ pg ++ e
F25H5.4 778 VKAYLPVNESFG-FTADLRSNTGGQAFPQCVFDHWQVLPG-DPLEA 821