Motif confirmation by HMMer
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
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HMM file: /home/niguts/usr02/tshini/ykclst/db/Pfam_fs
Sequence file: hmm2/seq/F32A5.6
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Query: F32A5.6 CE28290 status:Partially_confirmed SW:Q19951 protein_id:AAC46665.2
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
Peroxin-13_N Peroxin 13, N-terminal 311.8 8.3e-90 2
SH3 SH3 domain 53.3 5.3e-13 1
DUF561 Protein of unknown function (DUF561) 4.7 2.7 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
Peroxin-13_N 1/2 29 39 .. 1 11 [. -5.0 2.2e+03
DUF561 1/1 92 114 .. 236 258 .] 4.7 2.7
Peroxin-13_N 2/2 70 218 .. 1 167 [] 311.1 1.3e-89
SH3 1/1 238 298 .. 1 58 [] 53.3 5.3e-13
Alignments of top-scoring domains:
Peroxin-13_N: domain 1 of 2, from 29 to 39: score -5.0, E = 2.2e+03
*->GYnrgvalGmN<-*
G++ g+++GmN
F32A5.6 29 GFGYGGGYGMN 39
DUF561: domain 1 of 1, from 92 to 114: score 4.7, E = 2.7
*->ndieeMvnyiseikkaiskkrvl<-*
++ie+ vn++s ++ ++++++
F32A5.6 92 QSIESVVNAVSSVANMLNSTHNA 114
Peroxin-13_N: domain 2 of 2, from 70 to 218: score 311.1, E = 1.3e-89
*->GYnrgvalGmNlaesRFaqlaeesSraaFQsIESiVgAVgsvAqmLe
GY++g++ +N Fa+laee+Sr+aFQsIES+V+AV+svA+mL+
F32A5.6 70 GYGQGPD--SN-----FARLAEEQSRGAFQSIESVVNAVSSVANMLN 109
STfmAvhsSFravisVAeqFsrLKsslgsvfgifAvfrwLkylYRillkw
ST++Av+sSFravi+V+eqF+rLK++l+sv++++Avfrw+++++R w
F32A5.6 110 STHNAVYSSFRAVIGVVEQFGRLKTQLSSVVVSLAVFRWVYRFWR----W 155
lkrmlkLkPksfakkkefaealieefakkednvtekgaenrtrksvslwP
l++mlkLkP+s+a+++e+a++++++ ++t++++++rt++sv+ wP
F32A5.6 156 LLVMLKLKPASYASAAEMAWGTSQP------YATDVLGATRTPASVN-WP 198
lLlFlavvvGgPYLIiKLls<-*
++lF++v++GgP+LI++++s
F32A5.6 199 AALFWVVAIGGPWLIYRCVS 218
SH3: domain 1 of 1, from 238 to 298: score 53.3, E = 5.3e-13
*->pkvvALYDYeaeesdELsFkkGDvitvleksdd....gWWkgrlkgt
++++AL+D++a++++ELsF+ G+++ v k++ ++ +gW + + ++
F32A5.6 238 YTAQALFDFQASNEQELSFMNGETLRVAPKEEQprvrGWLLASVADG 284
gGkeGlvPsNYVepv<-*
+ + GlvP NYV+ v
F32A5.6 285 S-RIGLVPINYVRIV 298