Motif confirmation by HMMer
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
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HMM file: /home/niguts/usr02/tshini/ykclst/db/Pfam_fs
Sequence file: hmm2/seq/F35G12.2
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Query: F35G12.2 CE31500 isocitrate dehydrogenase status:Confirmed TR:Q20049 protein_id:CAA86325.2
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
isodh Isocitrate/isopropylmalate dehydrogenase 280.9 4.4e-83 1
NusB NusB family 5.0 5.9 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
isodh 1/1 53 381 .. 1 404 [] 280.9 4.4e-83
NusB 1/1 370 389 .. 115 134 .] 5.0 5.9
Alignments of top-scoring domains:
isodh: domain 1 of 1, from 53 to 381: score 280.9, E = 4.4e-83
*->kIavLpGDGIGPEvmeaalkVLkavgekaggelefefeeglvGgaai
+++vLpGDGIGPE+ + ++L av a+ +fe +++ +
F35G12.2 53 NVTVLPGDGIGPEMLHHVERILSAVQ--AP----VDFEVVNLTSKED 93
DetGiepLPeETleackeaCdavLlGAvgGPkggWDtltpkevRPEnsGL
+ E++ a+k++ ++L+G +++ ++ ++ + n
F35G12.2 94 ASED----LAEAITAIKRN-GVALKGNIETKF----DN--PSFVSRN--- 129
LaLRKeLdGLfANlRPvkvfspslgdaSPlkreiveggvDiviVRELTGG
L LR++L+ L+AN++ + + ++S+ g +D+vi+RE+T+G
F35G12.2 130 LELRRQLN-LYANVLHCSTI---PTVPSRHT-----G-IDMVIIRENTEG 169
iYfGipkgikggkklikflkeelvsgnfgeevavdtklYsrdEieRIarv
+Y+G ++++ +++ +v+ ++k+++r+ +e I r+
F35G12.2 170 EYSGNEHEA---------------VNAPHPRVVESLKVVTREKSEQITRF 204
AFelArkrgprkkVtsvdKaNVLksSrGAFKlWretveevakknkkskfe
AF+ A+k g rkkVt+v+KaN+ k+++G l+ ++++++ak+ e
F35G12.2 205 AFQFAKKYG-RKKVTAVHKANIQKLGDG---LFLKVATDIAKA------E 244
YPdveLeHmlvDnaamqLvknPkqFDfViVTeNlFGDILSDeASmltGSL
YPd+e++ m+vDna mqLv +P+qFD V +++Nl+G I+S A +l+G+
F35G12.2 245 YPDIEFNAMIVDNASMQLVSRPQQFD-VMLMPNLYGNIISNIACGLVGGP 293
GmlPSASlgaepdtsgfalyEPvHG...SAPDIAGKgkKGETSANPiAtI
G++ + ++g+ a++E G++++ + AGK+ ANP+A I
F35G12.2 294 GLVSGMNIGE-----DYAVFET--GtrnTGTTLAGKDL-----ANPTAFI 331
LSaAMMLRySlgleeaALdFdaiEaAVlktLeeGGirTgDLggeadGaky
+a+ MLR+ lgl +A d+i + tL + +i T+D+gg++
F35G12.2 332 RAAVDMLRF-LGLQSHA---DMISDSLFRTLVDKRIHTADIGGTS----- 372
vsTsemgdav<-*
+ se ++ v
F35G12.2 373 -KSSELVQSV 381
NusB: domain 1 of 1, from 370 to 389: score 5.0, E = 5.9
*->gddnsakFVNgVLrriakel<-*
g+++s+ +V +VL+ i+kel
F35G12.2 370 GTSKSSELVQSVLDFIEKEL 389