Motif confirmation by HMMer
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
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HMM file: /home/niguts/usr02/tshini/ykclst/db/Pfam_fs
Sequence file: hmm3/seq/K08B12.5
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Query: K08B12.5 CE30818 serine\/threonine-protein kinase status:Partially_confirmed TR:O01583 protein_id:AAB52260.3
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
CNH CNH domain 334.3 1.4e-96 1
pkinase Protein kinase domain 190.1 3.5e-53 1
DAG_PE-bind Phorbol esters/diacylglycerol binding 56.9 1.8e-13 1
PH PH domain 29.2 7.9e-07 1
pkinase_C Protein kinase C terminal domain 17.8 0.00032 1
PBD P21-Rho-binding domain 16.0 0.016 1
LCCL LCCL domain 5.2 3.7 1
MarR MarR family 4.2 7.9 1
PspA_IM30 PspA/IM30 family 4.0 7.4 1
MoCF_biosynth Probable molybdopterin binding domain 3.6 8.7 1
Mtap_PNP Phosphorylase family 2 3.0 6.5 1
V_ATPase_sub_a V-type ATPase 116kDa subunit family 1.5 8.4 1
Paramyxo_ncap Paramyxovirus nucleocapsid protein 1.2 8.1 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
V_ATPase_sub_a 1/1 195 205 .. 1 11 [. 1.5 8.4
pkinase 1/1 83 331 .. 1 273 [. 190.1 3.5e-53
pkinase_C 1/1 364 380 .. 15 31 .. 17.8 0.00032
PspA_IM30 1/1 577 616 .. 36 75 .. 4.0 7.4
Mtap_PNP 1/1 594 634 .. 241 281 .] 3.0 6.5
Paramyxo_ncap 1/1 852 867 .. 1 16 [. 1.2 8.1
DAG_PE-bind 1/1 958 1007 .. 1 51 [] 56.9 1.8e-13
LCCL 1/1 997 1014 .. 1 24 [. 5.2 3.7
PH 1/1 1027 1154 .. 1 85 [] 29.2 7.9e-07
MoCF_biosynth 1/1 1388 1413 .. 137 169 .] 3.6 8.7
MarR 1/1 1431 1460 .. 78 107 .] 4.2 7.9
CNH 1/1 1182 1479 .. 1 362 [] 334.3 1.4e-96
PBD 1/1 1544 1557 .. 1 14 [. 16.0 0.016
Alignments of top-scoring domains:
V_ATPase_sub_a: domain 1 of 1, from 195 to 205: score 1.5, E = 8.4
*->vsaLgelGlVq<-*
+++L++lG+V+
K08B12.5 195 IDSLHRLGYVH 205
pkinase: domain 1 of 1, from 83 to 331: score 190.1, E = 3.5e-53
*->yelleklGeGsfGkVykakhkdktgkiVAvKilkkekesikek....
+e+l+++G+G+fG+V ++ + + g+i+A+Kil+ k+++ ++ ++
K08B12.5 83 FEVLKVIGKGAFGEVAVVRMR-GVGEIYAMKILN--KWEMVKRaeta 126
rflrEiqilkrLsHpNIvrligvfedtddhlylvmEymegGdLfdylrrn
f+ E +l + I +l+++f+ ++++ly+vm+y gGd++++l++
K08B12.5 127 CFREERDVLVYGDRRWITNLHYAFQ-DEKNLYFVMDYYIGGDMLTLLSKF 175
ggplsekeakkialQilrGleYLHsngivHRDLKpeNILldendgtvKia
++++e ak+++ +++ +++ LH+ g vHRD+Kp+N+Lld + g++++a
K08B12.5 176 VDHIPESMAKFYIAEMVLAIDSLHRLGYVHRDVKPDNVLLDMQ-GHIRLA 224
DFGLArlle..sssklttfvGTpwYmmAPEvileg.....rgysskvDvW
DFG + + + s+ + vGTp+Y+ +PE+ l+ ++++ y++++D+W
K08B12.5 225 DFGSCLRILadGSVASNVAVGTPDYI-SPEI-LRAmedgrGRYGKECDWW 272
SlGviLyElltggplfpgadlpaftggdevdqliifvlklPfsdelpktr
SlG+ +yE+l+g ++Pf+ ++
K08B12.5 273 SLGICMYEMLYG--------------------------TTPFYS---ERL 293
idpleelfriikrp.glrlplpsncSeelkdLlkkcLn<-*
d++ ++++++ + +++ +++ +See+kdL++++++
K08B12.5 294 VDTYGKIMSHQDMLdFPDDEIDWVVSEEAKDLIRQLIC 331
pkinase_C: domain 1 of 1, from 364 to 380: score 17.8, E = 0.00032
*->PPFKPkiksprDtsNFD<-*
PP+ P+++sp+DtsNFD
K08B12.5 364 PPYVPEVSSPEDTSNFD 380
PspA_IM30: domain 1 of 1, from 577 to 616: score 4.0, E = 7.4
*->dmeselakarqalAqviArqkqlerkleeqkeqakklekk<-*
dm++ela+++++l + ++k+l++++e++k+q+++ e+k
K08B12.5 577 DMQAELADVGDSLLTEKDSVKRLQDEAEKAKKQVADFEEK 616
Mtap_PNP: domain 1 of 1, from 594 to 634: score 3.0, E = 6.5
*->eVlealkenknkahaevleaikqlaanelslicaillriAv<-*
++ + l+ ++ka+++v ++ ++l+ +e++ i++i ++++v
K08B12.5 594 DSVKRLQDEAEKAKKQVADFEEKLKEIETEKIALIKKQEEV 634
Paramyxo_ncap: domain 1 of 1, from 852 to 867: score 1.2, E = 8.1
*->mAsLLksLaLFKrnkd<-*
mAsL++ +a+ K++k+
K08B12.5 852 MASLMREVAMLKQQKN 867
DAG_PE-bind: domain 1 of 1, from 958 to 1007: score 56.9, E = 1.8e-13
*->HrFkrttfyksptfCdhCgellwglakQGlkCsnCglnvHkrChekV
H+F+r k+pt C hC ++l gl +QGl C+ C++ +H C+e+V
K08B12.5 958 HNFERMKI-KTPTKCGHCTSILIGLDRQGLFCQSCQYACHVSCAERV 1003
ptnC<-*
+ C
K08B12.5 1004 SQSC 1007
LCCL: domain 1 of 1, from 997 to 1014: score 5.2, E = 3.7
*->VtCdtrgldlcpHCfqkkanecnr<-*
V+C +r++++c ++ e++r
K08B12.5 997 VSCAERVSQSC------PVPEEER 1014
PH: domain 1 of 1, from 1027 to 1154: score 29.2, E = 7.9e-07
*->vikeGwLlkks.....kswkkRyfvLfndvLlyykdsk.........
+eG ++ ++ +k+w+ y+v+++ L++y+ +++++ ++
K08B12.5 1027 TAYEGLVKTPRaggvrKGWQTAYVVVCDFKLYLYDCTVdrqnkmqdv 1073
.kkpkgsipLsgcqvek.pd.............kncFeirt.dr......
+ + + ++++++ ++ ++ + + ++++ + F+++t++ +++++
K08B12.5 1074 kNEIRLVLDMRDPDFTVcGVseadvihaqkgdiPKIFRVTTtQIlnssse 1123
.......tlllqaeseeerkeWvkaiqsair<-*
+++++ l++ae+eee+++Wv a+++++
K08B12.5 1124 ysssskfYTLFMAETEEEKRKWVVALSELKT 1154
MoCF_biosynth: domain 1 of 1, from 1388 to 1413: score 3.6, E = 8.7
*->atggirggltkpvfgLPGnPvaalvafellvkP<-*
+++ k++++ PG+P l++f+ +++
K08B12.5 1388 YEA-------KLIVNVPGSPDEYLLVFNMIGLY 1413
[output cut off at A = 10 top alignments]