Motif confirmation by HMMer
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
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HMM file: /home/niguts/usr02/tshini/ykclst/db/Pfam_fs
Sequence file: hmm4/seq/R166.5
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Query: R166.5 CE03584 serine\/threonine kinase status:Partially_confirmed TR:Q22005 protein_id:CAA90665.1
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
pkinase Protein kinase domain 256.3 4e-73 2
CRAL_TRIO_N CRAL/TRIO, N-terminus 7.3 0.68 1
ATP-synt_ab_C ATP synthase alpha/beta chain, C term 5.2 3.1 1
Lipase_GDSL GDSL-like Lipase/Acylhydrolase 2.6 5.2 1
Cys-protease-3C 3C cysteine protease (picornain 3C) 2.5 3.9 1
DUF530 Protein of unknown function (DUF530) 2.2 4 1
Flavi_helicase Flavivirus helicase (NS3) 1.6 9.5 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
Cys-protease-3C 1/1 290 299 .. 174 183 .] 2.5 3.9
DUF530 1/1 281 307 .. 364 390 .. 2.2 4
Flavi_helicase 1/1 445 457 .. 304 316 .] 1.6 9.5
ATP-synt_ab_C 1/1 438 473 .. 100 147 .] 5.2 3.1
pkinase 1/2 201 480 .. 1 280 [. 256.1 4.7e-73
CRAL_TRIO_N 1/1 531 541 .. 79 89 .] 7.3 0.68
pkinase 2/2 536 547 .. 283 294 .] 0.2 56
Lipase_GDSL 1/1 550 599 .. 96 139 .. 2.6 5.2
Alignments of top-scoring domains:
Cys-protease-3C: domain 1 of 1, from 290 to 299: score 2.5, E = 3.9
*->LlRrYFteeQ<-*
L R+YFtee
R166.5 290 LQRKYFTEEE 299
DUF530: domain 1 of 1, from 281 to 307: score 2.2, E = 4
*->rggeLleelkkdlkvleevkkklskdv<-*
rgg Ll+ +++ ++ ee+ ++++kd+
R166.5 281 RGGPLLQHILQRKYFTEEEARRVTKDI 307
Flavi_helicase: domain 1 of 1, from 445 to 457: score 1.6, E = 9.5
*->IeDeekeIPersW<-*
I+D e+Pe++W
R166.5 445 IQDGYYEFPEEEW 457
ATP-synt_ab_C: domain 1 of 1, from 438 to 473: score 5.2, E = 3.1
*->tidlfyallrGklddlpedalekigtidiakykalnkellekakkll
+ dlf ++ +G+++ +pe+++ +i ++a ++ +ll
R166.5 438 QQDLFHRIQDGYYE-FPEEEWGMI--SEEA---------KDLVSNLL 472
k<-*
k
R166.5 473 K 473
pkinase: domain 1 of 2, from 201 to 480: score 256.1, E = 4.7e-73
*->yelleklGeGsfGkVykakhkdktgkiVAvKilkkekesikekrflr
+ e+lG+G++G+V +k + k+g ++AvKi++k+ e+ + kr+lr
R166.5 201 KLTDEHLGSGAYGSVTTCKSI-KSGVEYAVKIVDKQGETHSRKRILR 246
EiqilkrLs.HpNIvrligvfedtddhlylvmEymegGdLfdylrrnggp
E+ i k++++HpNIv+l+++fe +++++ylvmE m+gG L++++ +++ +
R166.5 247 EVNIFKTCKdHPNIVQLLDWFE-DETNFYLVMEKMRGGPLLQHILQRK-Y 294
lsekeakkialQilrGleYLHsngivHRDLKpeNILlden..dgtvKiaD
++e+ea++++++i +l+++H++gi HRD+KpeN+L++++++ vK++D
R166.5 295 FTEEEARRVTKDISLALKFMHDRGIAHRDVKPENVLCTDPnhVSPVKLCD 344
FGLArlle............sssklttfvGTpwYmmAPEvi...leg.rg
LA + +++++++ ++ s + l + vG+ ++m APEv++ + ++ +
R166.5 345 LDLASQRPpqherhplsqvaSEPDLASPVGSAEFM-APEVVdayVGDsLK 393
ysskvDvWSlGviLyElltggplfpga.dl.paftg.gdevdqliifvlk
y++k+D WSlGviLy +l g ++f+g d+++ +g + +
R166.5 394 YDKKCDTWSLGVILYIMLAGYAPFQGMcDDeD--CGwSE----------G 431
lPfsdelpktridpleelfriikrpglrlplpsncSeelkdLlkkcLnkD
P +d ++ d + ++ +++++ ++ + + +See+kdL++ +L++D
R166.5 432 KPCED----CQQDLFHRIQDGYYE--FPEEEWGMISEEAKDLVSNLLKRD 475
PskRp<-*
P +R+
R166.5 476 PVDRL 480
CRAL_TRIO_N: domain 1 of 1, from 531 to 541: score 7.3, E = 0.68
*->kWRrEfGvnei<-*
kWRr+f+ + i
R166.5 531 KWRRKFNADQI 541
pkinase: domain 2 of 2, from 536 to 547: score 0.2, E = 56
*->atakeilnhpwf<-*
++a +il h w+
R166.5 536 FNADQILSHRWL 547
Lipase_GDSL: domain 1 of 1, from 550 to 599: score 2.6, E = 5.2
*->sagAtIlp.ts..g.pf.l.iQv.qFkdfksqvlelrqalgllqell
sa++tIl+++s+ +++ + + v+q+ + + +++l++ + l+++
R166.5 550 SAASTILQtPSnlInRKdSaRDVqQMSEHFNLMNRLADTR-LSARFD 595
rllp<-*
++ +
R166.5 596 NKMT 599