Motif confirmation by HMMer
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
HMM file: /home/niguts/usr02/tshini/ykclst/db/Pfam_fs
Sequence file: hmm4/seq/T01E8.3
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: T01E8.3 CE02307 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase status:Partially_confirmed TR:Q22070 protein_id:CAA88745.1
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
PI-PLC-X Phosphatidylinositol-specific phospholipa 247.8 1.5e-70 1
PI-PLC-Y Phosphatidylinositol-specific phospholipa 160.5 2.8e-44 1
SH2 SH2 domain 136.4 5e-46 2
C2 C2 domain 99.5 2.9e-27 1
SH3 SH3 domain 31.8 4.3e-07 1
PH PH domain 29.1 8.8e-07 2
UQ_con Ubiquitin-conjugating enzyme 4.4 4.8 1
Nucleoporin Non-repetitive/WGA-negative nucleoporin 3.0 2.2 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
UQ_con 1/1 406 421 .. 1 16 [. 4.4 4.8
PI-PLC-X 1/1 302 453 .. 1 153 [] 247.8 1.5e-70
PH 1/2 480 510 .. 1 28 [. 10.4 0.17
SH2 1/2 554 635 .. 1 79 [] 76.2 3.7e-25
SH2 2/2 664 738 .. 1 79 [] 63.5 9.6e-21
SH3 1/1 784 837 .. 1 58 [] 31.8 4.3e-07
Nucleoporin 1/1 843 868 .. 1 26 [. 3.0 2.2
PH 2/2 810 909 .. 7 85 .] 18.7 0.00072
PI-PLC-Y 1/1 930 1042 .. 1 128 [] 160.5 2.8e-44
C2 1/1 1062 1152 .. 1 88 [] 99.5 2.9e-27
Alignments of top-scoring domains:
UQ_con: domain 1 of 1, from 406 to 421: score 4.4, E = 4.8
*->ssaatkRLqkElkelq<-*
s++a++ L++Elk+++
T01E8.3 406 SVPAQRLLAQELKDIL 421
PI-PLC-X: domain 1 of 1, from 302 to 453: score 247.8, E = 1.5e-70
*->dmsiPLsHYfisSshntYLtgkQlwGkssvesYrqqLdaGcRcvELD
dms+PLsHY+i+SshntYLtg+Ql+++ss Y q L++GcRc+ELD
T01E8.3 302 DMSRPLSHYWIASSHNTYLTGDQLRSESSLDCYAQALLMGCRCIELD 348
cwdGk.pdd.....epiIyHGhtltleiklkdVleaIkdfafkPtSpyPv
cwdG++ +++ + + +IyHG+t+t++i+l dVl+ I+ +af+ tS+yPv
T01E8.3 349 CWDGQkKPGsaefiDIVIYHGYTMTSKILLRDVLHVIRHYAFV-TSEYPV 397
IlSlenHcnsddqQrkmakyfkeiFgdmLltkPtldslttepglpLPslk
IlS+e++c+++ Qr a+ +k+i+gd+Llt P + ++ LPs+
T01E8.3 398 ILSIEDNCSVP-AQRLLAQELKDILGDYLLTQP-ANREEKQ----LPSPA 441
dlrgKILLknkk<-*
l+ KI++k+kk
T01E8.3 442 ALKKKIIVKHKK 453
PH: domain 1 of 2, from 480 to 510: score 10.4, E = 0.17
*->vikeGwLlkks...kswkkRyfvLfndvLly<-*
++k+G L++k +++ +w+ + f+Lf+d+L+y
T01E8.3 480 CVKKGILMVKNnnsHEWTSHVFILFPDRLCY 510
SH2: domain 1 of 2, from 554 to 635: score 76.2, E = 3.7e-25
*->WyhGkIsRkeAErlLlnp.ggkdGtFLvReSestpGdYtLSVrddgp
W+hG R eA ++ l+ +++ +G F++R+S +Gd++LS ++dg
T01E8.3 554 WFHGRCERDEAKKRILEHkEKGNGLFMIRDSNLFIGDFSLSILHDG- 599
rVkHyrIqs...ldnggyyitggvkFssLpeLvehY<-*
+V+H rI+s+ +++++yy ++++ +++L+eLv++Y
T01E8.3 600 KVHHVRIRSkiiDKEKKYYFMDNKVCDTLYELVSYY 635
SH2: domain 2 of 2, from 664 to 738: score 63.5, E = 9.6e-21
*->WyhGkIsRkeAErlLlnpggkdGtFLvReSestpGdYtLSVrddgpr
W+ +++AE+lL ++dG+FL+R S++ Y+LS++ dg +
T01E8.3 664 WFSATADKEKAEELLSL-VPEDGAFLIRTSSTDSSVYVLSLKVDG-E 708
VkHyrIqsldnggyyitggvkFssLpeLvehY<-*
+ Hyr +++++ ++ + F++L+++ve+Y
T01E8.3 709 FWHYRLKRDGR--IFVVNQKVFENLNQIVEFY 738
SH3: domain 1 of 1, from 784 to 837: score 31.8, E = 4.3e-07
*->pkvvALYDYeaeesdELsFkkGDvitvleksddgWWkgrlkgtgGke
+++AL +Y ++ +dELsF + +itvl k+++ W+gr+ + +
T01E8.3 784 VQARALRPYRGTADDELSFPANVIITVLRKEEG-LWRGRYGS---LT 826
GlvPsNYVepv<-*
G++Ps V+++
T01E8.3 827 GWFPSAHVQEI 837
Nucleoporin: domain 1 of 1, from 843 to 868: score 3.0, E = 2.2
*->stsgqglsepLelAgklieallerDr<-*
sts+ + + elAg lie+ Dr
T01E8.3 843 STSETSNYNTIELAGTLIERIHDADR 868
PH: domain 2 of 2, from 810 to 909: score 18.7, E = 0.00072
*->Llkks.kswkkRyfvLfndvLlyykdsk.............kkpkgs
+l+k+++ w+ Ry L++ ++ + + ++ +++ ++++++ + +
T01E8.3 810 VLRKEeGLWRGRYGSLTG----WFPSAHvqeilpekvstseTSNYNT 852
ipLsgcqvek.pd...kncFeirt.dr......tlllqaeseeerkeWvk
i L g +e+ +d +++n+++i+ ++++ +++ +ll+a+s+ee++ W +
T01E8.3 853 IELAGTLIERiHDadrPNVIRISQsNQhwmnkqYYLLAASSSEEADGWQN 902
aiqsair<-*
+ +++r
T01E8.3 903 NLFELTR 909
PI-PLC-Y: domain 1 of 1, from 930 to 1042: score 160.5, E = 2.8e-44
*->ElsnLvnYiqsikFrsFelsGeekntsyeisSFsErkvkakkllkes
ElsnLv Y+q + F + ++ye+ SF+E kl+k
T01E8.3 930 ELSNLVVYCQAVPFDPAHIY---NDAFYEMCSFVEG-----KLDKLV 968
pvefVkyNkrqLsRvYPkGtRvDSSNfmPqvfWnaGCQmVALNfQTsDlp
+ +N r LsRvYP G R S N++P+++WnaGC mVALN+QT D p
T01E8.3 969 EKGLLPFNSRKLSRVYPNGSRITSNNYSPVPMWNAGCHMVALNYQTGDKP 1018
mqiNdGmFeyNggqPdGsfksGYlLKPeflR<-*
mq+N+G+F++Ng+ +GYlLKP+ ++
T01E8.3 1019 MQLNQGKFLANGR-------CGYLLKPDYML 1042
C2: domain 1 of 1, from 1062 to 1152: score 99.5, E = 2.9e-27
*->LrVtvieAknLppkDkkngksDPYVkvslggdpkd.qkkkTkvikkt
L+V vi +++L kDk +g ++P+V++++ g+p d+++++Tk+i +
T01E8.3 1062 LNVQVIGGRHLSRKDKNKGICSPFVEIEIIGMPCDtKVFQTKTIASN 1108
.lNPvWNEtFtFevpppdlqetLeitVyDydrfgr..ddflGevs<-*
+lNP+WN+tFtFe+ +p+++ +++ V D d +g ++d f+G+++
T01E8.3 1109 gLNPIWNQTFTFEIQCPEVAL-IRFHVEDGDFVGPktDPFIGQAV 1152
[output cut off at A = 10 top alignments]