Motif confirmation by HMMer
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
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HMM file: /home/niguts/usr02/tshini/ykclst/db/Pfam_fs
Sequence file: hmm4/seq/T02G5.4
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Query: T02G5.4 CE04856 acetly-CoA thiolase status:Partially_confirmed TR:Q22106 protein_id:AAA82403.1
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
thiolase Thiolase, N-terminal domain 178.9 8e-50 2
thiolase_C Thiolase, C-terminal domain 157.8 1.9e-43 1
ketoacyl-synt Beta-ketoacyl synthase, N-terminal dom 6.8 0.87 1
Chal_stil_synt Chalcone and stilbene synthases, N-ter 4.1 3.8 1
TFIIE_alpha TFIIE alpha subunit 3.9 5 1
Cloacin_immun Cloacin immunity protein 3.6 8.1 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
TFIIE_alpha 1/1 25 57 .. 148 180 .] 3.9 5
ketoacyl-synt 1/1 189 219 .. 219 250 .. 6.8 0.87
Cloacin_immun 1/1 222 244 .. 60 82 .] 3.6 8.1
thiolase 1/2 99 252 .. 1 161 [. 170.5 2.8e-47
Chal_stil_synt 1/1 319 332 .. 215 228 .] 4.1 3.8
thiolase 2/2 320 332 .. 262 274 .] 11.2 0.042
thiolase_C 1/1 337 459 .. 1 142 [] 157.8 1.9e-43
Alignments of top-scoring domains:
TFIIE_alpha: domain 1 of 1, from 25 to 57: score 3.9, E = 5
*->DeedDnSkvikelkerlnrleeeifeslkalpk<-*
D +D +k++k+l + l+r+ +++ ++a++
T02G5.4 25 DNKVDEAKTTKDLDDVLERIQALVQSVIDAPKL 57
ketoacyl-synt: domain 1 of 1, from 189 to 219: score 6.8, E = 0.87
*->SSSlLvAlhlAvqslRrGecDlAlAGGVnlml<-*
SS+ A+ lA q++++G+ DlA+ GG + m+
T02G5.4 189 SSG-MKAIILAAQQIQTGHQDLAIGGGMESMS 219
Cloacin_immun: domain 1 of 1, from 222 to 244: score 3.6, E = 8.1
*->PyFknkidiskyeYfvsFdYRDG<-*
P+F +++ s ++Y+v DG
T02G5.4 222 PFFLARVEQSSNKYQVEQTVQDG 244
thiolase: domain 1 of 2, from 99 to 252: score 170.5, E = 2.8e-47
*->mkdVVIVsAaRTPiGKsfgGslkdlpAvdLgaiaikaaLeragippe
kd +IV+AaRTPiG sf+ sl++++A +L+++aikaaLer ++p+
T02G5.4 99 NKDAFIVGAARTPIG-SFRSSLSSVTAPELASVAIKAALERGAVKPS 144
pkaVde..vimGnVLqageaGqnpARqAaLlAGiPesvPaiTvNkvCgSG
++e +v +G+V a++ Gq+ ARqAaL+AG+ sv+ TvNk +SG
T02G5.4 145 --SIQEanVFLGQVCHANA-GQASARQAALGAGLDLSVAVTTVNKGWSSG 191
lkAvalaaQaIraGdaDivvAGGvEsMSnaPyvlprnaarsgarmGdakl
+kA++laaQ+I++G++D + GG+EsMS++P l r+ + +++
T02G5.4 192 MKAIILAAQQIQTGHQDLAIGGGMESMSQVPFFLA----RVEQSSNK-YQ 236
vDtmlkdgltdafnly<-*
v++ ++dg d+f+++
T02G5.4 237 VEQTVQDGISDFFDKQ 252
Chal_stil_synt: domain 1 of 1, from 319 to 332: score 4.1, E = 3.8
*->FGDGAAAvIiGaDP<-*
F DGAAAvI +
T02G5.4 319 FTDGAAAVILASQE 332
thiolase: domain 2 of 2, from 320 to 332: score 11.2, E = 0.042
*->nDGAAAvllMsEs<-*
+DGAAAv+l+s +
T02G5.4 320 TDGAAAVILASQE 332
thiolase_C: domain 1 of 1, from 337 to 459: score 157.8, E = 1.9e-43
*->lGlkplArivsyavvGvdPksimgigPvyAipkaLkkaGLtvnDiDl
+lkplAri +y+++++ ++ ++P++ p +L+ aG + +D+
T02G5.4 337 QNLKPLARILAYGDAATHQ-LDFAVAPTLLFPNLLQSAGVEQSDVAQ 382
fEiNEAFAaQaLavakaLgldpegkqmrdgdntyggkeKVNvnGGAIALG
+E+NEAF+ + La +k+Lg+dp + VN++GGA+++G
T02G5.4 383 WEVNEAFSCVPLAFIKKLGVDP---------------SLVNPHGGAVSIG 417
HPlGaSGARilvtLlheLkrrgggkyGlaalCiGGGqGvAmvver<-*
HP+G+SGA ++++L+h L+ g++G+aalC G G+ + mv+++
T02G5.4 418 HPIGMSGASLITHLVHTLES---GQIGVAALCDGDGGSSGMVIQK 459