Motif confirmation by HMMer
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
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HMM file: /home/niguts/usr02/tshini/ykclst/db/Pfam_fs
Sequence file: hmm4/seq/T04A8.7
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Query: T04A8.7 CE01076 1,4-Alpha-glucan branching enzyme status:Partially_confirmed TR:Q22137 protein_id:CAA84727.1
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
isoamylase_N Isoamylase N-terminal domain 94.0 5.4e-26 1
alpha-amylase Alpha amylase, catalytic domain 31.1 5.9e-08 2
alpha-amylase_C Alpha amylase, C-terminal all-beta do 6.6 1.3 1
GalP_UDP_transf Galactose-1-phosphate uridyl transfer 4.3 2.1 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
isoamylase_N 1/1 53 147 .. 1 121 [] 94.0 5.4e-26
alpha-amylase 1/2 212 269 .. 58 128 .. 31.1 6e-08
alpha-amylase 2/2 324 338 .. 237 250 .. -1.8 1.2e+02
GalP_UDP_transf 1/1 546 561 .. 1 16 [. 4.3 2.1
alpha-amylase_C 1/1 585 606 .. 1 25 [. 6.6 1.3
Alignments of top-scoring domains:
isoamylase_N: domain 1 of 1, from 53 to 147: score 94.0, E = 5.4e-26
*->yepLGahydgddtvdGevggvnFrvWAPnAerVsLvldfnnggkdwd
y+ +G ++++d ++v +WAP Ae+ +L++dfnn wd
T04A8.7 53 YKQFGLNVQPD-------NSVKGLEWAPAAEKLALIGDFNN----WD 88
geehpmkksetnrdgGvWevflpgd.nG.pdlphgsGgvrYkYrvdgpda
++++ +kk + G W++++p +++G++ +ph+s +k+ v +++
T04A8.7 89 QNANVYKK----EEHGKWSITVPAKeDGsCPIPHNS---VIKIAVSRHG- 130
pedeGhrfneiklklDPYAkavvgrp<-*
+ +kl+P+A++v++++
T04A8.7 131 ---------ATHFKLSPWATFVTCPN 147
alpha-amylase: domain 1 of 2, from 212 to 269: score 31.1, E = 6e-08
*->GvtaiWlsPifenipnhaspnkastsyhgYdttDYkkinQGvdpryG
G++ai+l+ + e h++ + +gY++++++++ +r G
T04A8.7 212 GYNAIQLMAVME----HVYY-----ASFGYQVSNFFAV----SSRCG 245
tkadfksLidaaharGIKvilDvV<-*
t +d+k L+d+ah++GI +lDvV
T04A8.7 246 TPEDLKYLVDKAHSLGIFMLLDVV 269
alpha-amylase: domain 2 of 2, from 324 to 338: score -1.8, E = 1.2e+02
*->dkGg.elGvDGFRiD<-*
+++++e+G+DGFR+D
T04A8.7 324 RWWVeEYGFDGFRFD 338
GalP_UDP_transf: domain 1 of 1, from 546 to 561: score 4.3, E = 2.1
*->kkfsplDhvhrRynpL<-*
+f+++D++++Ry+ L
T04A8.7 546 RQFNLVDAEYLRYKFL 561
alpha-amylase_C: domain 1 of 1, from 585 to 606: score 6.6, E = 1.3
*->tnWWdNGnnqiAFgRkfGnnkGfVA<-*
t W +G++ i+F+R G + +fV+
T04A8.7 585 TSWKHDGDKTIVFER--G-GLVFVI 606