Motif confirmation by HMMer
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
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HMM file: /home/niguts/usr02/tshini/ykclst/db/Pfam_fs
Sequence file: hmm4/seq/T14G8.3
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Query: T14G8.3 CE03659 heat shock protein status:Partially_confirmed TR:Q22515 protein_id:CAA91809.1
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
HSP70 Hsp70 protein 146.9 2.5e-42 5
UPF0169 Uncharacterised protein family (UPF0169) 8.7 0.4 1
NPD 2-nitropropane dioxygenase 2.0 8.3 1
SlyX SlyX 1.3 5 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
HSP70 1/5 50 72 .. 22 45 .. 2.4 4
HSP70 2/5 127 226 .. 100 202 .. 70.1 6.7e-20
UPF0169 1/1 296 317 .. 230 252 .] 8.7 0.4
SlyX 1/1 344 357 .. 1 14 [. 1.3 5
NPD 1/1 384 399 .. 393 408 .] 2.0 8.3
HSP70 3/5 260 429 .. 225 392 .. 91.2 4.8e-26
HSP70 4/5 524 613 .. 481 571 .. -16.7 5.9e+04
HSP70 5/5 645 751 .. 513 620 .] 2.3 4.3
Alignments of top-scoring domains:
HSP70: domain 1 of 5, from 50 to 72: score 2.4, E = 4
*->evIaNaeGnRtTPSvVAFtkkGER<-*
+++ N e++R TP v++F+++ ER
T14G8.3 50 DIVLNKESRRKTPNVISFKND-ER 72
HSP70: domain 2 of 5, from 127 to 226: score 70.1, E = 6.7e-20
*->veveylGkkktftPeeISAmvLqKmKetAEayLGekVtkAVITVPAY
v +y+++ +++ e Am+L K+ Eay +V+++VITVP +
T14G8.3 127 VVFPYKEE--KYNVETLLAMILWNAKKVTEAYADQTVKDVVITVPIF 171
FNDaQRQATKDAGkIAGLnVlRIiNEPTAAALAYGLDKkdlpe.eekern
N a+R+A+ A+ IAGLn l + N+ AAAL YG + + e ek+ +
T14G8.3 172 LNQAERRAIASAAEIAGLNLLQLLNDGSAAALNYGVFRRK--EiTEKPTH 219
ilVfDLG<-*
l++D+G
T14G8.3 220 MLIYDMG 226
UPF0169: domain 1 of 1, from 296 to 317: score 8.7, E = 0.4
*->lklgKQlkdeAkktakvlelNkp<-*
+++g l+ eA+++++vl +Nk+
T14G8.3 296 RAIG-KLHKEAERVKQVLSANKD 317
SlyX: domain 1 of 1, from 344 to 357: score 1.3, E = 5
*->eLeaRleeLEirlA<-*
eLe +e+LE r+A
T14G8.3 344 ELEKMIEDLEPRIA 357
NPD: domain 1 of 1, from 384 to 399: score 2.0, E = 8.3
*->deilsvkelIedLvqe<-*
+++++vke+++ ++++
T14G8.3 384 TRVPKVKEILKTILKD 399
HSP70: domain 3 of 5, from 260 to 429: score 91.2, E = 4.8e-26
*->GDThLGGeDFDnrlvdhlveeFkkk..egiDlskDkrAlqRLreAAE
+D+ LGG nrl dhl + F+ + ++ +D+s++ rA+ +L +AE
T14G8.3 260 FDKTLGGLEITNRLRDHLEKVFRDTvkTSKDISTNARAIGKLHKEAE 306
KAKieLSsssqTeinLpfitadadG...PkhlsetLtRAkFEeLvadLfe
++K +LS+ + ++ +++++++ + ++k ++ +tR+++E++++dL
T14G8.3 307 RVKQVLSA--NKDTYAQVESLFEEQnfrAKVINLLVTREELEKMIEDLEP 354
RtlePvekaLkDAklsksdIdeVvLVGGSTRIPkVqelVkefFGdGKePs
R+ P a+ A +s++dId VvL G TR+PkV+e++k ++ Ke s
T14G8.3 355 RIAAPILDAIGMAQISTNDIDLVVLMGAGTRVPKVKEILKTILK-DKEIS 403
kgvNPDEAVAiGAAvQaGVLsGseeV<-*
+N DEA+A+GA Qa+ Ls s++V
T14G8.3 404 NFLNTDEAIAMGAVYQAAHLSKSFKV 429
HSP70: domain 4 of 5, from 524 to 613: score -16.7, E = 5.9e+04
*->tFdIDANGIlnVsAkDkgtgkeqkItItnssgrLsddEIerMvkdAE
tF ID GI++V+ + k+ + ++ +++E + k++E
T14G8.3 524 TFSIDLSGIVSVEKASVVVEKVPTPEEKDKYE-VDKKEFDEWEKEQE 569
kyaeeDkkrkeriEakNeaEsyvystekslkeeklgdklseadk<-*
+ ++++k+ k++ E k + E + e++ eek ++k +e +k
T14G8.3 570 ELKKKEKAEKKEKEEKKKTEGEEEKNEDAAGEEKTEEKTEEVEK 613
HSP70: domain 5 of 5, from 645 to 751: score 2.3, E = 4.3
*->rLsddEIerMvkdAEkyaeeDkkrkeriEakNeaEsyvystekslke
+L+++E+ k + +++k++ +r Ea N++E ++y++ l++
T14G8.3 645 DLNEEEVTAAKKVLGDFEQKEKEKHDREEAMNRLEGLLYDLAVRLED 691
e.klgdklseadkekiekkvedaiewLkealegeddaekeeikakteeLq
+++++ ++e++k++i ++v w ++ + e kee+ +k+ +L+
T14G8.3 692 GeEYAEYATEEEKKAILEEVAVLKLWFEDDVSLET--KKEEFDEKRAKLE 739
kvvqkigekmyq<-*
+ k ++ ++
T14G8.3 740 ELTAKPNARKQE 751