Motif confirmation by HMMer
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
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HMM file: /home/niguts/usr02/tshini/ykclst/db/Pfam_fs
Sequence file: hmm4/seq/W02F12.5
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Query: W02F12.5 CE31083 dihydrolipoamide succinyltransferase status:Confirmed TR:O45148 protein_id:AAC04462.2
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
2-oxoacid_dh 2-oxo acid dehydrogenases acyltransfera 434.8 1.2e-127 1
biotin_lipoyl Biotin-requiring enzyme 85.5 1.3e-22 1
HlyD HlyD family secretion protein 10.7 0.11 1
GCV_H Glycine cleavage H-protein 8.3 0.56 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
GCV_H 1/1 83 118 .. 42 77 .. 8.3 0.56
biotin_lipoyl 1/1 64 136 .. 1 75 [] 85.5 1.3e-22
HlyD 1/1 104 136 .. 1 33 [. 10.7 0.11
2-oxoacid_dh 1/1 231 461 .. 1 233 [] 434.8 1.2e-127
Alignments of top-scoring domains:
GCV_H: domain 1 of 1, from 83 to 118: score 8.3, E = 0.56
*->LPEvGteVskgdslgavESVKaASevYaPvsGeVvE<-*
L+ +G+ V+ ++ ++++E K+ ev+aP G++vE
W02F12.5 83 LKQKGDHVNEDELVAEIETDKTSVEVPAPQAGTIVE 118
biotin_lipoyl: domain 1 of 1, from 64 to 136: score 85.5, E = 1.3e-22
*->keiksPmiGesvkegtPvaevlVkvGDkVkaGqvlcevEaMKmemei
+++ P++ es+ eg +++l++ GD+V++++ ++e+E++K ++e+
W02F12.5 64 ITVEGPAFAESISEG--DIRWLKQKGDHVNEDELVAEIETDKTSVEV 108
pApvaGvvkeilvkeGdtVevGdpLaki<-*
pAp+aG+++e lv++G++V++ + L k+
W02F12.5 109 PAPQAGTIVEFLVEDGAKVTAKQKLYKL 136
HlyD: domain 1 of 1, from 104 to 136: score 10.7, E = 0.11
*->rskevqpqvsGiVieilvkeGdrVkaGqvLvql<-*
s+ev ++ +G ++e lv++G++V a q L++l
W02F12.5 104 TSVEVPAPQAGTIVEFLVEDGAKVTAKQKLYKL 136
2-oxoacid_dh: domain 1 of 1, from 231 to 461: score 434.8, E = 1.2e-127
*->pgreervPlsgiRkaIAkrMteSkqtiPhftlfdevdmtkLlkLRkk
+++e rv+ +++R +IA+r++++++t +++t+f+e+dm++L+++Rk
W02F12.5 231 ARDEVRVKANRMRMRIAQRLKDAQNTYAMLTTFNEIDMSSLIEMRKT 277
lkd.faekhGvKLsfldFiiKAvakALkkfPevNAswdgdaneIvykkyv
++++f +khGvKL++++ +++A+a AL++ P+vNA +d++ eIvy+++v
W02F12.5 278 YQKdFVAKHGVKLGMMSPFVRAAAYALQESPVVNAVLDEN--EIVYRHFV 325
nIGvAVaTpnGLvVPViknAdkkslaeIakeikeLaekARegKLtpeEmq
+I+vAVaTp+GLvVPV++n++ ++ a+I+ e++ L kAR+gKL+ e+m
W02F12.5 326 DISVAVATPKGLVVPVLRNVESMNYAQIELELANLGVKARDGKLAVEDME 375
GGTFTISNlGmfGglffTPIINpPQvAILGvGaiekrPvvvdgeifvvrp
GGTFTISN+G+fG++f+TPIINpPQ+AILG+++ +r+v v+g+ ++rp
W02F12.5 376 GGTFTISNGGVFGSMFGTPIINPPQSAILGMHGVFDRVVPVNGKP-EIRP 424
mmylsLSyDHRvIDGaeAarFLkevKelLEdPellll<-*
+m+++L+yDHR+IDG+eA+ FLk++K +EdP+ +++
W02F12.5 425 IMQIALTYDHRLIDGREAVTFLKKIKTAVEDPRIMFM 461