Motif confirmation by HMMer
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
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HMM file: /home/niguts/usr02/tshini/ykclst/db/Pfam_fs
Sequence file: hmm5/seq/Y32F6A.3
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Query: Y32F6A.3 CE20204 POLY(A) POLYMERASE (EC 2.7.7.19) (PAP) (POLYNUCLEOTIDE ADENYLYLTRANSFERASE) status:Partially_confirmed TR:Q9U2P3 prot
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
PAP_central Poly(A) polymerase central domain 364.1 1.5e-105 1
PAP_RNA-bind Poly(A) polymerase predicted RNA binding 243.3 1.2e-71 1
NTP_transf_2 Nucleotidyltransferase domain 38.5 3.3e-09 1
DUF447 Protein of unknown function (DUF447) 4.2 4.9 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
DUF447 1/1 39 56 .. 185 204 .] 4.2 4.9
NTP_transf_2 1/1 84 166 .. 18 122 .] 38.5 3.3e-09
PAP_central 1/1 8 361 .. 1 205 [] 364.1 1.5e-105
PAP_RNA-bind 1/1 362 506 .. 1 212 [] 243.3 1.2e-71
Alignments of top-scoring domains:
DUF447: domain 1 of 1, from 39 to 56: score 4.2, E = 4.9
*->kkvGGereklleeielimrs<-*
kk G++++k+ e e++m++
Y32F6A.3 39 KKFGSYEPKE--ETEQRMEV 56
NTP_transf_2: domain 1 of 1, from 84 to 166: score 38.5, E = 3.3e-09
*->avaevylfGSyarGtylpgdSDIDllvvspdpvdr.ef.alffslar
a+ +++ fGSy+ G + g DID l v+p++ dr++f ++f
Y32F6A.3 84 AGGKLFTFGSYRLGVHSSG-ADIDTLAVVPRHIDRsDFfTSF----- 124
lgkrikevkrleelaeiekeleellglevDlv.vreklecilekkadplv
+e+ + el+g+e +v+v+++++ +v
Y32F6A.3 125 -----------KEMLNNDPNVTELHGVEEAFVpVMKLKY--------SGV 155
rielleervll<-*
++l+ r++l
Y32F6A.3 156 ELDILFARLAL 166
PAP_central: domain 1 of 1, from 8 to 361: score 364.1, E = 1.5e-105
RF xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
*->stkqyGvtkpislagpkekdvkltesLieeLkefgsf..........
+t+ +Gv++pisla+p++kd+++t+ Lie+Lk+fgs++++++++++
Y32F6A.3 8 KTPLLGVSQPISLAHPDSKDIAQTTLLIETLKKFGSYepkeeteqrm 54
RF
..................................................
+ ++ ++ ++ ++ + + +++++ + +++ + ++ + + ++++ +
Y32F6A.3 55 evlrnlnrlvkewvknvtamkipngegvnaggklftfgsyrlgvhssgad 104
RF
..................................................
++ +++ ++++ ++ ++ +++++ ++ ++ ++ + + + ++
Y32F6A.3 105 idtlavvprhidrsdfftsfkemlnndpnvtelhgveeafvpvmklkysg 154
RF xxxxx
.............................................eilkL
+ + + ++ +++++ ++++ ++ ++++ ++ ++ + +++lkL
Y32F6A.3 155 veldilfarlalkevpdtqelsddnllrnldqesvrslngcrvaeQLLKL 204
RF xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
VPnkyevFrltLraiKlWAkrrgiYsNvlGFlgGvaWAiLVAriCQlYPn
VP++ ++F++tLraiKlWAk++giYsN++GF+gG++WAiLVAr+CQlYPn
Y32F6A.3 205 VPRQ-KEFCVTLRAIKLWAKNHGIYSNSMGFFGGITWAILVARACQLYPN 253
RF xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
avpstlvekfFlvfsqWlrhnwpnPVlLkeinsdsieernlqvrvWdprk
a+ps+lv+++F++fs+W +wp+PV+L+e+n+d+ + +l++ vWdpr+
Y32F6A.3 254 ASPSRLVHRMFFIFSTW---TWPHPVVLNEMNNDRNDIPTLCELVWDPRR 300
RF xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
nksDricyhlmPiiTPAyPqqnstynVsestlkvileefkrgleildeie
+++Dr +h+mPiiTPA+P+qnst+nV++st++vi++e++++lei+++i+
Y32F6A.3 301 KNTDR--FHVMPIITPAFPEQNSTHNVTRSTATVIKNEICEALEICRDIS 348
RF xxxxxxxxxxxxx
lgkaeWskLfeka<-*
+gk++W++Lfe++
Y32F6A.3 349 EGKSKWTALFEEV 361
PAP_RNA-bind: domain 1 of 1, from 362 to 506: score 243.3, E = 1.2e-71
*->nFFerYkhYLqitasAagHmAEtaedhLkWsGlVESRlRlLvlkLEr
nFF+rYkh++++ ++A+ ++e++L+++G+ ESR+RlLv++LEr
Y32F6A.3 362 NFFSRYKHFIALIMAAP-----NEEEELNYGGFLESRIRLLVQSLER 403
negItlADYRQLRLHpnPkkFedtkecsvdeeaeniangygleksetrls
n++I +A H++P+k++ +++ ++d+++en+
Y32F6A.3 404 NQDIIIA-------HNDPNKHKPSPNAKFDVNPENK-------------- 432
alklAsdekkdeesskdenkavkdssaksgptvfttvwFIGLefkegvks
++tvwFIGLef+e+ k+
Y32F6A.3 433 ---------------------------------RVTVWFIGLEFAEHAKT 449
deekklDitypiqeFknickevnrqasnvkglkancqmeidikhVKrkqL
lD+t++iq+Fk ++v+ qasnvkg+++ncq++id+++VKr++L
Y32F6A.3 450 -----LDLTNEIQRFK---TNVELQASNVKGIGPNCQVQIDMFYVKRNSL 491
pqyvfedglkrpkks<-*
+q++++ +l+r++++
Y32F6A.3 492 IQVISAADLRRGRRW 506