Motif confirmation by HMMer
hmmpfam - search a single seq against HMM database
HMMER 2.1.1 (Dec 1998)
Copyright (C) 1992-1998 Washington University School of Medicine
HMMER is freely distributed under the GNU General Public License (GPL).
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
HMM file: /home/niguts/usr02/tshini/ykclst/db/Pfam_fs
Sequence file: hmm5/seq/Y45F10A.6b
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: Y45F10A.6b CE18397 status:Partially_confirmed TR:Q95NR8 protein_id:CAC42378.1
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
TBC TBC domain 180.0 3.1e-51 1
GRAM GRAM domain 178.7 1.1e-50 2
Plasmid_stabil Plasmid stabilization system protein 6.2 3.1 1
TBSV_P22 TBSV core protein P21/P22 3.2 5 1
DUF123 Domain of unknown function DUF123 3.1 7.6 1
PFK Phosphofructokinase 0.7 7.2 1
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
Plasmid_stabil 1/1 78 100 .. 65 88 .] 6.2 3.1
TBSV_P22 1/1 146 195 .. 128 179 .] 3.2 5
GRAM 1/2 166 233 .. 1 86 [] 98.6 2.6e-27
PFK 1/1 265 279 .. 298 312 .] 0.7 7.2
GRAM 2/2 321 389 .. 1 86 [] 81.6 2.3e-22
DUF123 1/1 526 539 .. 1 14 [. 3.1 7.6
TBC 1/1 509 721 .. 1 341 [] 180.0 3.1e-51
Alignments of top-scoring domains:
Plasmid_stabil: domain 1 of 1, from 78 to 100: score 6.2, E = 3.1
*->shyvvyYrlsgetgVlIlRVlHqr<-*
++y++ Y +++e+ V+I Vl +
Y45F10A.6b 78 PPYRIIYDFDDED-VHISIVLAVA 100
TBSV_P22: domain 1 of 1, from 146 to 195: score 3.2, E = 5
*->EELvsPylEGRELFsvslRWqlGLsAqsYsLPqvnvKvlYqEdalka
EE+vsPy E EL svs R l +s+s+P+ + v Y l +
Y45F10A.6b 146 EEVVSPYSE--ELDSVSTRSCLEKFHKSFSIPPDEKLVNYYKCCLWK 190
Lkysk<-*
k
Y45F10A.6b 191 GKVPA 195
GRAM: domain 1 of 2, from 166 to 233: score 98.6, E = 2.6e-27
*->ekfrkhFkeilppeEkLiadckkeVtysCplqrsfreeskipvqGrl
ekf+k F+ +pp+EkL+++ y+C+l+++ k+p+qG l
Y45F10A.6b 166 EKFHKSFS--IPPDEKLVNY------YKCCLWKG-----KVPAQGDL 199
YlTnnrlcFrSdllgdltkegviklviPladiesvekek<-*
+l+ n+lcF+++++g++t k+ ++++di+++e+++
Y45F10A.6b 200 FLSVNFLCFHAFMMGNET-----KIKLKWTDIVRLERVS 233
PFK: domain 1 of 1, from 265 to 279: score 0.7, E = 7.2
*->LASrmGaeAVeaLLe<-*
LAS+++ A ++L+e
Y45F10A.6b 265 LASQLANIAMKQLIE 279
GRAM: domain 2 of 2, from 321 to 389: score 81.6, E = 2.3e-22
*->ekfrkhFkeilppeEkLiadckkeVtysCplqrsfreeskipvqGrl
++fr++F+ lp +EkL++d + C l++++ ++++v+G+l
Y45F10A.6b 321 DAFRCQFN--LPLTEKLDGD------TQCRLFTPY---DRRHVPGKL 356
YlTnnrlcFrSdllgdltkegviklviPladiesvekek<-*
+++ n++cF+S ++ l+ ++v+Pl +++s+e ++
Y45F10A.6b 357 FVSANFVCFAS-RTERLV-----SIVVPLIEVTSIEECS 389
DUF123: domain 1 of 1, from 526 to 539: score 3.1, E = 7.6
*->kgklgeveFkrGYY<-*
g++ e+++ +GYY
Y45F10A.6b 526 SGAAAEMALNPGYY 539
TBC: domain 1 of 1, from 509 to 721: score 180.0, E = 3.1e-51
*->vrqgvpsslRgkVWklllgaqelnnclltdnfkgldlfglvpvllla
++gvp lRg++W +++ga + +
Y45F10A.6b 509 LLEGVPLQLRGQIWMVCSGAAAE---------------------MAL 534
dkdeYeellnknkektvqdqneKssvgirrldyVEAVEKHPLSDDNDKTK
++++Y+ell kn ++++ +
Y45F10A.6b 535 NPGYYRELLHKNQGVYSVA------------------------------- 553
GSLekgsdekalklredldkIekDlsRTfpdeiffqtrlaeqqlkkdqdl
l++Ie+Dl+R++p+ ++fq
Y45F10A.6b 554 -----------------LEEIERDLHRSLPEHPAFQ-------------- 572
daydkDEfddeddkneppsikqLrrlLvaYswknpqehlgYvQGMnvils
+p+i++Lrr+L+aY+ np+ +gY+Q+Mn+++s
Y45F10A.6b 573 ---------------QGPGIDALRRILTAYAFRNPN--IGYCQAMNIVGS 605
pLLlflkhgvdldeideeqAFwclvkLmdnylpqkyflndlsglnedlrv
+LLlf ee AFw+lv+ +++ lp+ y++ g+ d++v
Y45F10A.6b 606 VLLLFT---------KEEEAFWLLVAVCERLLPD-YYNTKVVGALVDQGV 645
LdslvkeslPeLyshlkkkenktgsgkKknllaldltllifafpwfLtlF
+++lv+ +lP + l + +l+ + +a++wfLt+F
Y45F10A.6b 646 FSELVERLLPTVGAQLTRL---------------GLD-DMVALSWFLTVF 679
arelPleivlrIwDilftyYlgshflifvalAiLkllkskllkh<-*
+ + ++rI+D +f++ g ++ ++val Lk ++ + ++
Y45F10A.6b 680 LSAIKFDAAVRILDLFFFE--GARLMFQVALEMLKENEKLICES 721